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Analyst. 2013 Mar 21;138(6):1653-6. doi: 10.1039/c3an36545j. Epub 2013 Feb 4.

Suspended microtubules demonstrate high sensitivity and low experimental variability in kinesin bead assay.

Author information

1
Center for International Research on Micronano Mechatronic, Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Tokyo 153-8505, Japan. mctarhan@iis.u-tokyo.ac.jp

Abstract

Microtubule (MT) based intraneuronal transport deficiency is directly linked to neurodegeneration. Hence, the development of a reliable and sensitive in vitro approach permitting efficient analysis of MT-based transport is essential for our understanding of the underlying molecular mechanisms that may lead to novel therapeutic approaches for treating neurodegenerative diseases. Here, based on previously developed reconstructed MT-kinesin assay, we propose its "suspended" modification that shows higher sensitivity and lower experimental variability.

PMID:
23376984
DOI:
10.1039/c3an36545j
[Indexed for MEDLINE]

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