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Diagn Microbiol Infect Dis. 2013 Apr;75(4):381-9. doi: 10.1016/j.diagmicrobio.2012.12.005. Epub 2013 Feb 1.

Rapid Chagas diagnosis in clinical settings using a multiparametric assay.

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1
Byo Research S.r.l., Area Science Park, 16305, Basovizza, Trieste, Italy.

Abstract

The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings.

[Indexed for MEDLINE]

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