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Hepatol Res. 2013 Oct;43(10):1105-14. doi: 10.1111/hepr.12062. Epub 2013 Feb 3.

Derangement of ghrelin secretion after long-term high-fat diet feeding in rats.

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1
Faculty of Pharmacy, Keio University, Tokyo, Japan.

Abstract

AIM:

Appetite control is an important goal for the management of non-alcoholic fatty liver disease, diabetes mellitus and obesity; however, little is known about how hormones concerning appetite regulation are affected by long-term consumption of a high-fat diet. We investigated the effect of high-fat diet on secretory regulation of ghrelin and leptin in rats.

METHODS:

Rats were fed a control or a high-fat diet for 18 weeks and then killed. Before being killed, a glucose tolerance test was performed. Weight, total calorie intake and blood glucose levels were measured, and the plasma levels of total and active ghrelin, and leptin were analyzed by enzyme-linked immunosorbent assay.

RESULTS:

Body and fat weight and total calorie intake were significantly higher in the high-fat diet group than in the control, although blood glucose levels did not differ. Plasma leptin was significantly higher in the high-fat diet group, and a significant positive correlation was observed between bodyweight and leptin levels in both groups. The levels of active and total ghrelin were not significantly changed by high-fat diet, and active ghrelin levels in the control group significantly correlated negatively with bodyweight, while its correlation was lost in the high-fat diet group. The glucose tolerance test showed that ghrelin levels were significantly higher than those of controls even 60 min after glucose loading.

CONCLUSION:

These results indicate that secretion of ghrelin, but not leptin, are deranged by consumption of a high-fat diet, and active ghrelin levels lose their correlation with bodyweight and food intake.

KEYWORDS:

appetite; calorie; central obesity; leptin; non-alcoholic steatohepatitis

PMID:
23374505
DOI:
10.1111/hepr.12062
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