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Biomacromolecules. 2013 Mar 11;14(3):834-40. doi: 10.1021/bm3018802. Epub 2013 Feb 15.

Electrochemiluminescence biosensor based on conducting poly(5-formylindole) for sensitive detection of Ramos cells.

Author information

1
State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China. gmnie@126.com

Abstract

A signal-on electrochemiluminescence (ECL) biosensor devoted to the detection of Ramos cells was fabricated based on a novel conducting polymer, poly(5-formylindole) (P5FIn), which was synthesized electrochemically by direct anodic oxidation of 5-formylindole (5FIn). This ECL platform was presented by covalently coupling the 18-mer amino-substituted oligonucleotide (ODN) probes with aldehyde groups that are strongly reactive toward a variety of nucleophiles on the surface of solid substrates. The specific identification and high-affinity between aptamers and target cells, gold nanoparticles (AuNPs) enhanced ECL nanoprobes, along with P5FIn induced ECL quenching contributed greatly to the sensitivity and selectivity. The ECL signals were logarithmically linear with the concentration of Ramos cells in a wide determination range from 500 to 1.0 × 10(5) cells mL(-1), and the corresponding detection limit was 300 cells mL(-1).

PMID:
23373751
DOI:
10.1021/bm3018802
[Indexed for MEDLINE]

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