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Org Lett. 2013 Feb 15;15(4):756-9. doi: 10.1021/ol303328t. Epub 2013 Jan 31.

Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.

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1
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu City 30010, Taiwan.

Abstract

We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

PMID:
23368695
PMCID:
PMC3681297
DOI:
10.1021/ol303328t
[Indexed for MEDLINE]
Free PMC Article
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