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Muscle Nerve. 2013 Apr;47(4):522-31. doi: 10.1002/mus.23640. Epub 2013 Jan 31.

NF-KB activity functions in primary pericytes in a cell- and non-cell-autonomous manner to affect myotube formation.

Author information

  • 1Department of Kinesiology, University of Massachusetts Amherst, 300 Massachusetts Avenue, Amherst, Massachusetts 01003, USA. robhyldahl@byu.edu

Abstract

INTRODUCTION:

Skeletal muscle regeneration following damage relies on proliferation and differentiation of muscle precursor cells (MPCs). We recently observed increased NF-kB activity in vascular-associated muscle resident pericytes following muscle damage in humans. We determined how altered NF-kB activity in human primary pericytes (HPPs) affects their myogenic differentiation (cell-autonomous effects), as well as proliferation and differentiation of co-cultured MPCs (non-cell-autonomous effects).

METHODS:

HPPs were transfected with vectors that increased or decreased NF-kB activity. Transfected HPPs were co-cultured with C2 C12 myoblasts under differentiation conditions, and HPP fusion to myotubes was measured. We also co-cultured HPPs with C2 C12 myoblasts and measured proliferation and myotube formation.

RESULTS:

Inhibition of NF-kB activity increased HPP fusion to C2 C12 myotubes. Moreover, enhanced NF-kB activity in HPPs suppressed differentiation and enhanced proliferation of co-cultured myoblasts.

CONCLUSIONS:

NF-kB activity acts cell-autonomously to inhibit HPP myogenic differentiation and non-cell-autonomously to promote MPC proliferation and suppress MPC differentiation in vitro.

PMID:
23364895
DOI:
10.1002/mus.23640
[PubMed - indexed for MEDLINE]
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