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J Clin Microbiol. 2013 Apr;51(4):1281-4. doi: 10.1128/JCM.00190-13. Epub 2013 Jan 30.

Direct sequencing and RipSeq interpretation as a tool for identification of polymicrobial infections.

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1
The Danish Technological Institute, Life Science Division, Aarhus, Denmark.

Abstract

In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

PMID:
23363825
PMCID:
PMC3666778
DOI:
10.1128/JCM.00190-13
[Indexed for MEDLINE]
Free PMC Article
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