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J Clin Microbiol. 2013 Apr;51(4):1066-70. doi: 10.1128/JCM.00093-13. Epub 2013 Jan 30.

Whole-genome mapping: a new paradigm in strain-typing technology.

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1
Microbiology Technical Services, LLC, Dunwoody, Georgia, USA. jmm8@comcast.net

Abstract

Strain-typing technology in support of outbreak identification and resolution has evolved from phenotypic analysis, such as serology and biotypes, to much-more-robust molecular genetic approaches, such as pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing. Whole-genome mapping (WGM) has been recently applied to subtyping analysis, and it bridges the gap between PFGE (∼20 bands sorted by size) and whole-genome sequencing. WGM utilizes restriction site analysis but arranges 200 to 500 bands in the order they appear on the chromosome. WGM is able to quickly and cost-effectively generate high-resolution, ordered whole-genome maps of bacteria.

PMID:
23363821
PMCID:
PMC3666824
DOI:
10.1128/JCM.00093-13
[Indexed for MEDLINE]
Free PMC Article
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