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Nano Lett. 2013 Feb 13;13(2):781-5. doi: 10.1021/nl304492y. Epub 2013 Feb 5.

DNA origami nanopillars as standards for three-dimensional superresolution microscopy.

Author information

1
Institut für Physikalische und Theoretische Chemie, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106 Braunschweig, Germany.

Abstract

Nanopillars are promising nanostructures composed of various materials that bring new functionalities for applications ranging from photovoltaics to analytics. We developed DNA nanopillars with a height of 220 nm and a diameter of ~14 nm using the DNA origami technique. Modifying the base of the nanopillars with biotins allowed selective, upright, and rigid immobilization on solid substrates. With the help of site-selective dye labels, we visualized the structure and determined the orientation of the nanopillars by three-dimensional fluorescence superresolution microscopy. Because of their rigidity and nanometer-precise addressability, DNA origami nanopillars qualify as scaffold for the assembly of plasmonic devices as well as for three-dimensional superresolution standards.

PMID:
23362960
DOI:
10.1021/nl304492y
[Indexed for MEDLINE]

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