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Biomol NMR Assign. 2014 Apr;8(1):133-5. doi: 10.1007/s12104-013-9468-4. Epub 2013 Jan 30.

¹H, ¹⁵N and ¹³C assignments of a putative peptidyl prolyl cis-trans isomerase FKBP12 from Trypanosoma brucei.

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1
Instituto de Bioquímica Médica, CCS, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowiski s/n, Rio de Janeiro, RJ, 21941-590, Brazil.

Abstract

TbFKBP12 is a putative peptidyl prolyl cis-trans isomerase from Trypanosoma brucei, causative agent of the African trypanosomiasis or sleeping sickness. It interacts with the immunosuppressive drug rapamycin inhibiting the formation of TORC2 complex leading to parasite death by inhibiting cell proliferation through cytokinesis blockade. Moreover, RNAi silencing of TbFKBP12 revealed essential function in both procyclic and bloodstream forms. Both facts make TbFKBP12 an attractive target for ligand development and thus structural data is desirable. In this work we report the NMR resonance assignments for (1)H, (15)N and (13)C nuclei in the backbone and side chains of the TbFKBP12 as basis for further studies of structure, backbone dynamics, interaction mapping and drug screening.

PMID:
23361379
DOI:
10.1007/s12104-013-9468-4
[Indexed for MEDLINE]
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