Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Biotechnol. 2013 Mar;31(3):230-2. doi: 10.1038/nbt.2507. Epub 2013 Jan 29.

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

Author information

1
National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea.

Abstract

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

Comment in

PMID:
23360966
DOI:
10.1038/nbt.2507
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center