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Nat Biotechnol. 2013 Mar;31(3):230-2. doi: 10.1038/nbt.2507. Epub 2013 Jan 29.

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

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1
National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea.

Abstract

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

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PMID:
23360966
DOI:
10.1038/nbt.2507
[Indexed for MEDLINE]

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