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Sci Rep. 2013;3:1146. doi: 10.1038/srep01146. Epub 2013 Jan 28.

Efficacy of post exposure administration of doxycycline in a murine model of inhalational melioidosis.

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1
Battelle, Battelle Memorial Institute, Columbus, OH, USA. gelhaush@battelle.org

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. Treatment of melioidosis is suboptimal and developing improved melioidosis therapies requires animal models. In this report, we exposed male BALB/c mice to various amounts of aerosolized B. pseudomallei 1026b to determine lethality. After establishing a median lethal dose (LD(50)) of 2,772 colony forming units (cfu)/animal, we tested the ability of doxycycline administered 6 hours after exposure to a uniformly lethal dose of ~20 LD(50) to prevent death and eliminate bacteria from the lung and spleens. Tissue bacterial burdens were examined by PCR analysis. We found that 100% of mice treated with doxycycline survived and B. pseudomallei DNA was not amplified from the lungs or spleens of most surviving mice. We conclude the BALB/c mouse is a useful model of melioidosis. Furthermore, the data generated in this mouse model indicate that doxycycline is likely to be effective in post-exposure prophylaxis of melioidosis.

PMID:
23359492
PMCID:
PMC3556592
DOI:
10.1038/srep01146
[Indexed for MEDLINE]
Free PMC Article
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