Abstract
Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated products identified them as faithful copies of the hepatitis delta virus genome found in virions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Viral / analysis
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cell Line
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Cells, Cultured
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DNA Replication*
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DNA, Viral / genetics
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Fibroblasts / metabolism
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Genes, Viral*
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Hepatitis Delta Virus / genetics*
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Liposomes
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Mice
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Molecular Sequence Data
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RNA, Viral / administration & dosage
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RNA, Viral / chemical synthesis
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RNA, Viral / genetics*
Substances
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Antigens, Viral
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DNA, Viral
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Liposomes
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RNA, Viral