Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Oral Implants Res. 2014 Mar;25(3):366-71. doi: 10.1111/clr.12112. Epub 2013 Jan 28.

Morphological evidences of Bio-Oss® colonization by CD44-positive cells.

Author information

1
Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Granada, Spain.

Abstract

OBJECTIVES:

Our objectives were to examine vascular and cellular colonization in anorganic bovine bone (ABB) after 6 months of healing in human maxillary sinus augmentation grafts.

MATERIAL AND METHODS:

Fifty unilateral maxillary sinus augmentation procedures were performed on 50 consecutive patients. Bone cores were obtained through the implant receptor sites 6 months later and evaluated with morphological image analysis and immunohistochemical techniques.

RESULTS:

Image analysis revealed a mean of 35.44 ± 16% vital bone, 31.66 ± 15% non-mineralized tissue, and 32.72 ± 25% remnant ABB particles. In our patients, neovascularization in 46.3% of cases was demonstrated within ABB particles 6 months after sinus floor augmentation surgery. Neovascularization of ABB particles was inversely related to age, directly to osteoclast number per mm², and not influenced by habits or disease. CD44-positive cells colonization was found in 74% of cases. An important correlation was found regarding CD44 expression and number of vessel in ABB particles (r = 0.624 P < 0.001, Pearson). Osteopontin expression was detected on the interstitial boundary of bone with ABB particles and within the osteocyte lacunae and bone canaliculi and was relationship with presence of CD44-positive cells inside ABB particles (r = 0.388, P = 0.046, Pearson).

CONCLUSION:

In conclusion, images compatible with osteone colonized by osteocytes CD44 positive and neovascularization in ABB particles were observed after 6 months of graft maturation. These biologic events have not previously been morphologically and immunohistochemically documented.

KEYWORDS:

CD44; anorganic bovine bone; bone remodeling; intrasinus graft; neovascularization; osteocyte; osteopontin

PMID:
23356496
DOI:
10.1111/clr.12112
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center