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Appl Biochem Biotechnol. 2013 Mar;169(6):1910-8. doi: 10.1007/s12010-012-9992-1. Epub 2013 Jan 25.

Heterologous expression and characterization of a glycoside hydrolase family 45 endo-β-1,4-glucanase from a symbiotic protist of the lower termite, Reticulitermes speratus.

Author information

1
Biomass Research Platform Team, Biomass Engineering Program, Research Cluster for Innovation, RIKEN, 1-7-29 Suehiro-cho, Tsurumi-ward, Yokohama, Kanagawa, Japan. masatoo@riken.jp

Abstract

The termite symbiotic system is one of the efficient lignocellulose degradation systems. We tried to express and characterize a novel cellulolytic enzyme from this system. Here, we report the isolation of an endo-β-1,4-glucanase gene homolog of glycoside hydrolase family 45 from a symbiotic protistan community of Reticulitermes speratus. Heterologous expression of this gene was performed using the expression system of Aspergillus oryzae. Analysis of enzymatic properties revealed 786 μmol/min/mg protein in specific activity, a V max of 833.0 units/mg protein, and a K m value of 2.58 mg/ml with carboxymethyl cellulose as the substrate. Thin-layer chromatography analysis showed that RsSymEG2 produces cellobiose from cellodextrins larger than cellohexaose. This enzyme showed high specific activity like other endo-β-1,4-glucanases from the symbiotic system of termites. It means that the termite symbiotic system is a good resource for highly active endo-β-1,4-glucanases.

PMID:
23354496
DOI:
10.1007/s12010-012-9992-1
[Indexed for MEDLINE]

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