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J Toxicol Pathol. 2012 Dec;25(4):273-6. doi: 10.1293/tox.25.273. Epub 2012 Dec 20.

Splenic hemangiosarcoma in a young sprague-dawley rat.

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Drug Safety Research Laboratories, Astellas Pharma Inc., 1-6 Kashima 2-chome, Yodogawa-ku, Osaka 532-8514, Japan.


The present report describes a rare case of spontaneous hemangiosarcoma in a nine-week-old male Sprague-Dawley rat. At necropsy, multiple white nodules of various sizes were observed on and within the enlarged spleen and liver and were histopathologically determined to be composed of spindle- to oval-shaped cells that showed invasive growth without encapsulation and were arranged solidly but partially in whorls or faint alveolar patterns with vascular-like spaces containing small clefts or erythrocytes in the tumor mass. Immunohistochemical analysis revealed that most of the tumor cells were strongly positive for vimentin, von Willebrand factor (vWF) and CD34 but negative for podoplanin. In addition, electron microscopic examination revealed the presence of Weibel-Palade bodies in the cytoplasm of the tumor cells. Based on these findings, this case was diagnosed as a hemangiosarcoma. The splenic masses were larger than the hepatic ones, with tumor cells mainly observed at periportal regions with tumor embolism in the liver, suggesting that primary hemangiosarcoma initially developed in the spleen before metastasizing.


Weibel-Palade bodies; hemangiosarcoma; rat; spontaneous; young

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