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J Cell Sci. 2013 Mar 1;126(Pt 5):1176-88. doi: 10.1242/jcs.116327. Epub 2013 Jan 23.

Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway.

Author information

1
Department of Immunology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.

Abstract

A major outstanding issue in cell biology is the lack of understanding of the contribution of tubulovesicular transport carriers (TCs) to intracellular trafficking pathways within 3D cellular environments. This is primarily due to the challenges associated with the use of microscopy techniques to track these highly motile, small compartments. In the present study we have used multifocal plane microscopy with localized photoactivation to overcome these limitations. Using this approach, we have characterized individual components constituting the recycling pathway of the receptor FcRn. Specifically, several different pathways followed by TCs that intersect with larger, relatively static sorting endosomes have been defined. These pathways include a novel 'looping' process in which TCs leave and return to the same sorting endosome. Significantly, TCs with different itineraries can be identified by associations with distinct complements of Rab GTPases, APPL1 and SNX4. These studies provide a framework for further analyses of the recycling pathway.

PMID:
23345403
PMCID:
PMC3635461
DOI:
10.1242/jcs.116327
[Indexed for MEDLINE]
Free PMC Article

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