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Angew Chem Int Ed Engl. 2013 Feb 25;52(9):2440-3. doi: 10.1002/anie.201206828. Epub 2013 Jan 22.

Rapid characterization of hydrogen exchange in proteins.

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1
NMR Research Centre, Indian Institute of Science, Bangalore-560012, India.

Abstract

Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional (13)CO-(15)N NMR correlation experiments. The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen-deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.

PMID:
23341333
DOI:
10.1002/anie.201206828
[Indexed for MEDLINE]

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