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Mol Syst Biol. 2013;9:641. doi: 10.1038/msb.2012.66.

Genome-scale engineering for systems and synthetic biology.

Author information

1
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA. kevin.esvelt@wyss.harvard.edu

Abstract

Genome-modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre-defined locations across the genome. The handful of methods capable of targeted gene editing suffered from low efficiencies, significant labor costs, or both. Recent advances have dramatically expanded our ability to engineer cells in a directed and combinatorial manner. Here, we review current technologies and methodologies for genome-scale engineering, discuss the prospects for extending efficient genome modification to new hosts, and explore the implications of continued advances toward the development of flexibly programmable chasses, novel biochemistries, and safer organismal and ecological engineering.

PMID:
23340847
PMCID:
PMC3564264
DOI:
10.1038/msb.2012.66
[Indexed for MEDLINE]
Free PMC Article
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