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J Dermatol Sci. 2013 Mar;69(3):187-94. doi: 10.1016/j.jdermsci.2012.12.006. Epub 2013 Jan 5.

Association between collagen production and mechanical stretching in dermal extracellular matrix: in vivo effect of cross-linked hyaluronic acid filler. A randomised, placebo-controlled study.

Author information

1
Centre de Recherche sur la Peau Pierre Fabre, Hôtel Dieu, Toulouse, France. virginie.turlier@pierre-fabre.com

Abstract

BACKGROUND:

The effects of hyaluronic acid (HA) injection on tissue collagen anabolism are suggested to be related to the induction of mechanical stress, causing biochemical changes in skin physiology.

OBJECTIVES:

To ascertain the association between dermal mechanics modulated by a hyaluronic acid-based filler effect and metabolism.

METHODS:

Sixty females were randomised to receive a 0.5mL injection of HA gel or isotonic sodium chloride (control) in the arm. Skin biopsies were taken at baseline and after 1, 3 and 6 months. Protein and gene expression of procollagen, matrix metalloproteinases (MMP) and MMP tissue inhibitors (TIMP1) were measured blind by ELISA and qPCR, respectively. Injected volumes were measured by high-frequency ultrasound and radiofrequency analysis. Skin layer effects of injections were analysed by finite element digital modelling.

RESULTS:

One month after injection, the filler induced an increase in procollagen (p=0.0016) and TIMP-1 (p=0.0485) levels and relative gene expression of procollagen III and I isoforms compared with the controls. After 3 months, procollagen levels remained greater than in the controls (p=0.0005), whereas procollagen expression and TIMP-1 and MMP content were no longer different. Forty-three percent of the injected filler volume was found at 1 month, 26% after 3 months and 20% after 6 months.

LIMITATIONS:

The ultrasound imaging technique limited the scope of the investigation and precluded an evaluation of the action of the filler at the hypodermic level.

CONCLUSIONS:

Integrating both mechanical and biological aspects, our results suggest that mechanical stress generated by cross-linked HA plays a role in dermal cell biochemical response.

PMID:
23340440
DOI:
10.1016/j.jdermsci.2012.12.006
[Indexed for MEDLINE]

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