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Appl Environ Microbiol. 2013 Apr;79(7):2121-7. doi: 10.1128/AEM.03528-12. Epub 2013 Jan 18.

Characterization of a novel metagenome-derived 6-phospho-β-glucosidase from black liquor sediment.

Author information

1
State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.

Abstract

The enzyme 6-phospho-β-glucosidase is an important member of the glycoside hydrolase family 1 (GH1). However, its catalytic mechanisms, especially the key residues determining substrate specificity and affinity, are poorly understood. A metagenome-derived gene sequence, encoding a novel 6-phospho-β-glucosidase designated Pbgl25-217, was isolated and characterized. The optimal conditions for enzymatic activity were 37°C and pH 7; Ca(2+), Mg(2+), and Mn(2+) stabilized the activity of Pbgl25-217, whereas Ni(2+), Fe(2+), Zn(2+), Cu(2+), and Fe(3+) inhibited its activity. The Km and Vmax of Pbgl25-217 were 4.8 mM and 1,987.0 U mg(-1), respectively. Seven conserved residues were recognized by multiple alignments and were tested by site-directed mutagenesis for their functions in substrate recognition and catalytic reaction. The results suggest that residues S427, Lys435, and Tyr437 act as "gatekeepers" in a phosphate-binding loop and play important roles in phosphate recognition. This functional identification may provide insights into the specificity of 6-phospho-β-glycosidases in GH1 and be useful for designing further directed evolution.

PMID:
23335769
PMCID:
PMC3623223
DOI:
10.1128/AEM.03528-12
[Indexed for MEDLINE]
Free PMC Article

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