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J Biosci Bioeng. 2013 Jun;115(6):691-4. doi: 10.1016/j.jbiosc.2012.12.020. Epub 2013 Jan 18.

Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

Author information

1
Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.

Abstract

Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.

PMID:
23333644
DOI:
10.1016/j.jbiosc.2012.12.020
[Indexed for MEDLINE]

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