Format

Send to

Choose Destination
Dev Cell. 2013 Jan 28;24(2):182-95. doi: 10.1016/j.devcel.2012.12.008. Epub 2013 Jan 17.

Actin filament elongation in Arp2/3-derived networks is controlled by three distinct mechanisms.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.

Abstract

Spatial and temporal control of actin filament barbed end elongation is crucial for force generation by actin networks. In this study, genetics, cell biology, and biochemistry were used to reveal three complementary mechanisms that regulate actin filament barbed end elongation in Arp2/3-derived networks. Aip1 inhibits elongation of aged ADP-actin filaments decorated with cofilin and, together with capping protein (CP), maintains a high level of assembly-competent actin species. We identified Abp1 and Aim3 as two additional proteins that work together to inhibit barbed end elongation. Abp1/Aim3 collaborates with CP to control elongation of newly assembled ATP-actin filaments to organize filament polarity within actin networks. Thus, three distinct mechanisms control filament elongation in different regions of Arp2/3 networks, maintaining pools of assembly-competent actin species while ensuring proper filament polarity and facilitating force production.

PMID:
23333351
PMCID:
PMC3562429
DOI:
10.1016/j.devcel.2012.12.008
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center