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Elife. 2012 Dec 31;1:e00248. doi: 10.7554/eLife.00248.

rsEGFP2 enables fast RESOLFT nanoscopy of living cells.

Author information

1
Department of NanoBiophotonics , Max Planck Institute for Biophysical Chemistry , Göttingen , Germany.

Abstract

The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001.

KEYWORDS:

GFP; None; confocal microscopy; fluorescent probes; live-cell imaging; nanoscopy; superresolution

PMID:
23330067
PMCID:
PMC3534202
DOI:
10.7554/eLife.00248
[Indexed for MEDLINE]
Free PMC Article

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