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Methods Mol Biol. 2013;960:145-157. doi: 10.1007/978-1-62703-218-6_12.

Biochemical large-scale identification of MHC class I ligands.

Author information

1
Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.
2
Department of Immunology, University of Tübingen, Interfaculty Institute for Cell Biology, Tübingen, Germany. stefan.stevanovic@uni-tuebingen.de.

Abstract

The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this chapter we briefly review the existing strategies for the analysis of MHC ligandomes and provide an in-depth protocol for the immunoaffinity purification of MHC class I presented peptides from primary tissues or cells.

PMID:
23329485
DOI:
10.1007/978-1-62703-218-6_12
[Indexed for MEDLINE]

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