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World J Gastroenterol. 2012 Dec 28;18(48):7285-9. doi: 10.3748/wjg.v18.i48.7285.

Growth-inhibitory effects of MOB2 on human hepatic carcinoma cell line SMMC-7721.

Author information

1
Institute of Hepatobiliary Surgery, PLA General Hospital, Beijing 100853, China.

Abstract

AIM:

To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.

METHODS:

The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells. The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1. After lipofection of the MOB2 gene into cancer cells, the levels of MOB2 protein in the cancer cells were detected by immunoblotting. To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells, the cells were cultured in Dulbecco's Modified Eagle's Medium with 10% fetal calf serum and glutamine, and then mixed with liposomes, Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2.

RESULTS:

We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry. We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2. After transfection, MOB2 enhanced growth suppression, induced apoptosis, increased the ratio of G0/G1, significantly inhibited the advance of cell cycle phase, and arrested cells in G0/G1 phase.

CONCLUSION:

MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells, which may be useful in gene therapy for hepatic carcinoma.

KEYWORDS:

Apoptosis; Gene expression; Growth inhibition; SMMC-7721

PMID:
23326134
PMCID:
PMC3544031
DOI:
10.3748/wjg.v18.i48.7285
[Indexed for MEDLINE]
Free PMC Article
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