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World J Gastroenterol. 2012 Dec 28;18(48):7158-65. doi: 10.3748/wjg.v18.i48.7158.

Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells.

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1
The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.

Abstract

AIM:

To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.

METHODS:

Male Sprague-Dawley rats underwent common bile duct ligation (BDL) for 14 d and were treated with Gardenia jasminoides by gavage. The effects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells (LX-2) in vitro.

RESULTS:

Treatment with Gardenia jasminoides decreased serum alanine aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 146.6 ± 15 U/L vs 77 ± 6.5 U/L, P = 0.0007) and aspartate aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 188 ± 35.2 U/L vs 128 ± 19 U/L, P = 0.005) as well as hydroxyproline (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue, P = 0.004) after BDL. Furthermore, Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1 (TGF-β1), collagen type I (Col I) and α-smooth muscle actin (α-SMA). Gardenia jasminoides significantly suppressed the upregulation of TGF-β1, Col I and α-SMA in LX-2 exposed to recombinant TGF-β1. Moreover, Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.

CONCLUSION:

Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.

KEYWORDS:

Collagen type I; Gardenia jasminoides; Liver fibrosis; Transforming growth factor-β1/Smad2 pathway; α-smooth muscle actin

PMID:
23326120
PMCID:
PMC3544017
DOI:
10.3748/wjg.v18.i48.7158
[Indexed for MEDLINE]
Free PMC Article
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