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Methods Mol Biol. 2013;961:17-32. doi: 10.1007/978-1-62703-227-8_2.

Molecular diagnosis of autoimmune blistering diseases.

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Department of Dermatology, School of Medicine, and Institute of Cutaneous Cell Biology, Kurume University, Kurume, Fukuoka, Japan.


Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1-3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purified proteins from cell cultures and recombinant proteins.

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