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Korean J Radiol. 2013 Jan-Feb;14(1):30-7. doi: 10.3348/kjr.2013.14.1.30. Epub 2012 Dec 28.

In vitro and in vivo imaging of prostate cancer angiogenesis using anti-vascular endothelial growth factor receptor 2 antibody-conjugated quantum dot.

Author information

1
Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K), Seongnam 463-400, Korea.

Abstract

OBJECTIVE:

Authors aimed to determine the targeting ability of vascular endothelial growth factor receptor 2 (VEGFR2)-conjugated quantum dots (QDs) in vitro, and apply it for a xenograft prostate cancer mouse model.

MATERIALS AND METHODS:

Conjugation reaction of QDs was performed by using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and sulfo-(N-hydroxysulfosuccinimide) (Sulfo-NHS). The human umbilical vein cord endothelial cells (HUVECs) were incubated with QDs, conjugated with antiVGFR2, to see a specific binding in vitro. Fluorescent cell images were taken by a confocal microscope. The human prostate cancer cells (PC3) were injected to five nude mice on hind limbs to make the xenograft tumor model. QD-antiVEGFR2 antibody complex was injected into the tumor model and fluorescence measurements were performed at 1, 4, 9, 12, 15, and 24 hours after the injection.

RESULTS:

The specific interaction between HUVECs and QD-antiVEGFR2 antibody was clearly shown in vitro. The in vivo fluorescence image disclosed that there was an increased signal of tumor, 12 hours after the injection of QDs.

CONCLUSION:

By showing endothelial cells binding with QDs-antiVEGFR2 antibodyand an experimental application of the antibody for VEGFR2 imaging in the prostate cancer xenograft mouse model, we suggests that the antibody-conjugated QDs can be a potential imaging tool for angiogenesis of the cancer.

KEYWORDS:

Angiogenesis; Near infrared; Prostate cancer; Quantum dot; VEGFR2

PMID:
23323028
PMCID:
PMC3542300
DOI:
10.3348/kjr.2013.14.1.30
[Indexed for MEDLINE]
Free PMC Article

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