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J Proteomics. 2013 Feb 21;79:231-50. doi: 10.1016/j.jprot.2012.12.023. Epub 2013 Jan 9.

Proteomic analysis of the flooding tolerance mechanism in mutant soybean.

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1
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

Abstract

Flooding stress of soybean is a serious problem because it reduces growth; however, flooding-tolerant cultivars have not been identified. To analyze the flooding tolerance mechanism of soybean, the flooding-tolerant mutant was isolated and analyzed using a proteomic technique. Flooding-tolerance tests were repeated five times using gamma-ray irradiated soybeans, whose root growth (M6 stage) was not suppressed even under flooding stress. Two-day-old wild-type and mutant plants were subjected to flooding stress for 2days, and proteins were identified using a gel-based proteomic technique. In wild-type under flooding stress, levels of proteins related to development, protein synthesis/degradation, secondary metabolism, and the cell wall changed; however, these proteins did not markedly differ in the mutant. In contrast, an increased number of fermentation-related proteins were identified in the mutant under flooding stress. The root tips of mutant plants were not affected by flooding stress, even though the wild-type plants had damaged root. Alcohol dehydrogenase activity in the mutant increased at an early stage of flooding stress compared with that of the wild-type. Taken together, these results suggest that activation of the fermentation system in the early stages of flooding may be an important factor for the acquisition of flooding tolerance in soybean.

PMID:
23313221
DOI:
10.1016/j.jprot.2012.12.023
[Indexed for MEDLINE]
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