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J Am Chem Soc. 2013 Jan 30;135(4):1406-14. doi: 10.1021/ja3091615. Epub 2013 Jan 22.

Enzyme molecules as nanomotors.

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1
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.

Abstract

Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher substrate concentration, a form of chemotaxis at the molecular scale. Using glucose oxidase and glucose to generate a hydrogen peroxide gradient, we induce the migration of catalase toward glucose oxidase, thereby showing that chemically interconnected enzymes can be drawn together.

PMID:
23308365
DOI:
10.1021/ja3091615
[Indexed for MEDLINE]
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