Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2013;8(1):e53689. doi: 10.1371/journal.pone.0053689. Epub 2013 Jan 7.

Detection of cyclic diguanylate G-octaplex assembly and interaction with proteins.

Author information

1
Department of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, Maryland, United States of America.

Erratum in

  • PLoS One. 2013;8(6). doi:10.1371/annotation/1e2e5c25-79ad-4cf2-b619-f6972eafbadc.

Abstract

Bacterial signaling networks control a wide variety of cellular processes including growth, metabolism, and pathogenesis. Bis-(3'-5')-cyclic dimeric guanosine monophosphate (cdiGMP) is a secondary signaling nucleotide that controls cellulose synthesis, biofilm formation, motility and virulence in a wide range of gram-negative bacterial species. CdiGMP is a dynamic molecule that forms different tertiary structures in vitro, including a trans-monomer, cis-monomer, cis-dimer and G-octaplex (G8). Although the monomer and dimer have been shown to be physiologically relevant in modulating protein activity and transcription, the biological effects of the cdiGMP G8 has not yet been described. Here, we have developed a TLC-based assay to detect radiolabeled cdiGMP G8 formation. Utilizing the radiolabeled cdiGMP G8, we have also shown a novel inhibitory interaction between the cdiGMP G8 and HIV-1 reverse transcriptase and that the cdiGMP G8 does not interact with proteins from Pseudomonas aeruginosa known to bind monomeric and dimeric cdiGMP. These results suggest that the radiolabeled cdiGMP G8 can be used to measure interactions between the cdiGMP G8 and cellular proteins, providing an avenue through which the biological significance of this molecule could be investigated.

PMID:
23308275
PMCID:
PMC3538687
DOI:
10.1371/journal.pone.0053689
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center