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Bioresour Technol. 2013 Feb;129:592-8. doi: 10.1016/j.biortech.2012.11.099. Epub 2012 Dec 1.

Liquid hot water pretreatment of sugarcane bagasse and its comparison with chemical pretreatment methods for the sugar recovery and structural changes.

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Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.


Liquid hot water (LHW), dilute hydrochloric acid (HCl) and dilute sodium hydroxide (NaOH) were applied to sugarcane bagasse (SB). Application of the same analytical methods and material balance approaches facilitated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated sugarcane bagasse. Adding Tween80 in the enzymatic hydrolysis process increased the conversion level of glucan/xylan by 0.3-fold, especially for the low pH pretreatment where more lignin was left in the solids. The total sugar recovery from sugarcane bagasse with the coupled operations of pretreatment and 72 h enzymatic digestion reached 71.6% for LHW process, 76.6% for HCl pretreatment and 77.3% for NaOH pretreatment. Different structural changes at the plant tissue, cellular, and cell wall levels might be responsible for the different enzymatic digestibility. Furthermore, a combined LHW and aqueous ammonia pretreatment was proposed to reduce energy input and enhance the sugar recovery.

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