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J Biol Eng. 2013 Jan 10;7(1):2. doi: 10.1186/1754-1611-7-2.

2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts.

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Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.


There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

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