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J Neurosci. 2013 Jan 9;33(2):678-86. doi: 10.1523/JNEUROSCI.1404-12.2013.

LTP-induced long-term stabilization of individual nascent dendritic spines.

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1
Center for Neuroscience, University of California Davis, Davis, California 95618, USA.

Abstract

Learning new tasks has been associated with increased growth and stabilization of new dendritic spines. We examined whether long-term potentiation (LTP), a key cellular mechanism thought to underlie learning, plays a role in selective stabilization of individual new spines during circuit plasticity. Using two-photon glutamate uncaging, we stimulated nascent spines on dendrites of rat hippocampal CA1 neurons with patterns that induce LTP and then monitored spine survival rates using time-lapse imaging. Remarkably, we found that LTP-inducing stimuli increased the long-term survivorship (>14 h) of individual new spines. Activity-induced new spine stabilization required NMDA receptor activation and was specific for stimuli that induced LTP. Moreover, abrogating CaMKII binding to the NMDA receptor abolished activity-induced new spine stabilization. Our findings demonstrate for the first time that, in addition to enhancing the efficacy of preexisting synapses, LTP-inducing stimuli promote the transition of nascent spines from a short-lived, transient state to a longer-lived, persistent state.

PMID:
23303946
DOI:
10.1523/JNEUROSCI.1404-12.2013
[Indexed for MEDLINE]
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