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Chem Commun (Camb). 2013 Feb 14;49(13):1312-4. doi: 10.1039/c2cc38174e. Epub 2013 Jan 10.

Femtomolar Zn(II) affinity of minimal zinc hook peptides--a promising small tag for protein engineering.

Author information

1
Laboratory of Chemical Biology, University of Wrocław, ul. Tamka 2, 50-137 Wrocław, Poland.

Abstract

The minimal zinc hook peptide of Rad50 and its alanine mutants form highly stable Zn(II) complexes. These peptides were successfully used as a small, efficient tag for reversible Zn(II)-mediated protein homodimerization. The high stability, its biological consequences and potential applications in protein engineering are discussed.

PMID:
23303248
DOI:
10.1039/c2cc38174e
[Indexed for MEDLINE]

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