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J Microsc. 2013 Mar;249(3):184-94. doi: 10.1111/jmi.12008. Epub 2013 Jan 10.

New image colocalization coefficient for fluorescence microscopy to quantify (bio-)molecular interactions.

Author information

1
Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany. hdherce@gmail.com

Abstract

The spatial relationship, or degree of colocalization, between two or more types of molecules in live cells is commonly detected using fluorescence microscopy. This spatial distribution can be used to estimate the interaction between fluorescently labelled molecules. These interactions are usually quantified by analysing the correlation and/or the overlap between images, using the Pearson's and Manders' coefficients, respectively. However, the correlation and overlap coefficients are parameters not designed to quantify molecular interactions. Here we propose a new colocalization coefficient specifically designed to quantify the interactions between molecules. In well-defined thermodynamic ensembles, this coefficient can in principle be used to calculate relevant statistical thermodynamic quantities such as binding free energies.

PMID:
23301670
PMCID:
PMC3599484
DOI:
10.1111/jmi.12008
[Indexed for MEDLINE]
Free PMC Article
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