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PLoS One. 2012;7(12):e52627. doi: 10.1371/journal.pone.0052627. Epub 2012 Dec 27.

Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.

Author information

1
Membrane and Cytoskeleton Dynamics, Institut Curie, Research Center, CNRS- UMR144, Paris, France. maud.hertzog@ibcg.biotoul.fr

Erratum in

  • PLoS One. 2013;8(8). doi:10.1371/annotation/fc7791c5-45e2-44ae-868b-f0529c56033f.

Abstract

Caveolae are specialized domains of the plasma membrane, which play key roles in signaling, endocytosis and mechanosensing. Using total internal reflection fluorescent microscopy (TIRF-M), we observe that the exocyst subunit Exo70 forms punctuate structures at the plasma membrane and partially localizes with caveolin-1, the main component of caveolae. Upon cell detachment, we found that Exo70 accumulates with caveolin-1-positive vesicular structures. Upon cell re-adhesion, caveolin-1 traffics back to the plasma membrane in a multistep process involving microtubules and actin cytoskeleton. In addition, silencing of Exo70 redirects caveolin-1 to focal adhesions identified by markers such as α5 integrin or vinculin. Based on these findings, we conclude that Exo70 is involved in caveolin-1 recycling to the plasma membrane during re-adhesion of the cells to the substratum.

PMID:
23300727
PMCID:
PMC3531403
DOI:
10.1371/journal.pone.0052627
[Indexed for MEDLINE]
Free PMC Article
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