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Methods Mol Biol. 2013;966:77-95. doi: 10.1007/978-1-62703-245-2_5.

Visualization of Gram-positive bacterial pili.

Author information

1
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, TX, USA. Chungyu.Chang@uth.tmc.edu

Abstract

Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.

PMID:
23299729
DOI:
10.1007/978-1-62703-245-2_5
[Indexed for MEDLINE]

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