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Bioresour Technol. 2013 Feb;129:639-41. doi: 10.1016/j.biortech.2012.12.074. Epub 2012 Dec 20.

Evaluation of a transgenic poplar as a potential biomass crop for biofuel production.

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1
School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.

Abstract

A transgenic poplar, in which the RabG3bCA gene from Arabidopsis was overexpressed, was analyzed for its biomass composition and enzymatic digestibility after chemical pretreatment. In comparison with a wild-type poplar (WT), the transgenic poplar (OX8) showed 9.8% higher glucan content. The levels of other biomass components did not differ greatly between WT and OX8. When WT and OX8 samples were pretreated by sulfuric acid (1%, w/v at 190 °C), sodium hydroxide (1%, w/v at 190 °C), or ammonia (14%, w/w at 80 °C), the washed pretreated solids of OX8 exhibited a higher enzymatic digestibility than those of WT in each chemical pretreatment. The sodium hydroxide pretreatment was the most effective among the three pretreatment processes, showing 58.7% and 69.4% of theoretical glucose yield from the saccharification of pretreated OX8 and WT, respectively. The transgenic poplar, growing faster and taller, was found to contain more glucan and have a higher enzymatic digestibility than WT.

PMID:
23298768
DOI:
10.1016/j.biortech.2012.12.074
[Indexed for MEDLINE]

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