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FEMS Microbiol Rev. 2013 May;37(3):407-27. doi: 10.1111/1574-6976.12015. Epub 2013 Feb 11.

The future is now: single-cell genomics of bacteria and archaea.

Author information

1
Broad Institute of Harvard and MIT, Cambridge, MA, USA. pblainey@mit.edu

Abstract

Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology.

PMID:
23298390
PMCID:
PMC3878092
DOI:
10.1111/1574-6976.12015
[Indexed for MEDLINE]
Free PMC Article
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