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Methods Mol Biol. 2013;971:227-44. doi: 10.1007/978-1-62703-269-8_13.

FISH and FICTION to detect chromosomal aberrations in lymphomas.

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1
Institute of Human Genetics, University Hospital Schleswig-Holstein, Christian-Albrechts University, Kiel, Germany. mgiefing@medgen.uni-kiel.de

Abstract

Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique allowing the visualization of target sequences like genes in interphase nuclei. It is widely used in routine diagnostics to identify cancer specific aberrations including lymphoma associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping-a technique called Fluorescence Immunophenotyping and Interphase Cytogenetic as a Tool for Investigation Of Neoplasia (FICTION)-it is moreover possible to identify a cell population of interest. Here we describe standard protocols for FISH and FICTION as used in our laboratory in diagnosis and research.

PMID:
23296967
DOI:
10.1007/978-1-62703-269-8_13
[Indexed for MEDLINE]
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