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Methods Mol Biol. 2013;968:195-201. doi: 10.1007/978-1-62703-257-5_15.

Multiplex-tandem PCR for fungal diagnostics.

Author information

1
Centre for Infectious Diseases and Microbiology, The University of Sydney, Sydney, NSW, Australia. anna.lau@nih.gov

Abstract

Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal pathogens in blood cultures, primary isolation plates, and whole blood, serum, and plasma.

PMID:
23296896
DOI:
10.1007/978-1-62703-257-5_15
[Indexed for MEDLINE]

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