Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2013;964:15-24. doi: 10.1007/978-1-62703-251-3_2.

Methods for the study of dopamine receptors within lipid rafts of kidney cells.

Author information

1
Department of Pediatrics, Center for Molecular Physiology Research, Children's National Medical Center, School of Medicine, George Washington University, Washington, DC, USA.

Abstract

There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D(1)-like receptors (D(1)R and D(5)R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non--caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.

PMID:
23296775
PMCID:
PMC4067094
DOI:
10.1007/978-1-62703-251-3_2
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Support Center