Format

Send to

Choose Destination
Methods Mol Biol. 2013;965:463-72. doi: 10.1007/978-1-62703-239-1_30.

Assessing chronological aging in Saccharomyces cerevisiae.

Author information

1
Ethel Percy Andrus Gerontology Center, Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.

Abstract

Saccharomyces cerevisiae is one of the most studied model organisms for the identification of genes and mechanisms that affect aging. The chronological lifespan (CLS) assay, which monitors the survival of a non-dividing population, is one of the two methods to study aging in yeast. To eliminate potential artifacts and identify genes and signaling pathways that may also affect aging in higher eukaryotes, it is important to determine CLS by multiple methods. Here, we describe these methods as well as the assays to study macromolecular damage during aging in yeast, with a focus on genomic instability.

PMID:
23296677
PMCID:
PMC4041521
DOI:
10.1007/978-1-62703-239-1_30
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center