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Methods Mol Biol. 2013;963:215-35. doi: 10.1007/978-1-62703-230-8_14.

Phage display selection of peptides that target calcium-binding proteins.

Author information

1
Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND, USA. stefan.vetter@ndsu.edu

Abstract

Phage display allows to rapidly identify peptide sequences with binding affinity towards target proteins, for example, calcium-binding proteins (CBPs). Phage technology allows screening of 10(9) or more independent peptide sequences and can identify CBP binding peptides within 2 weeks. Adjusting of screening conditions allows selecting CBPs binding peptides that are either calcium-dependent or independent. Obtained peptide sequences can be used to identify CBP target proteins based on sequence homology or to quickly obtain peptide-based CBP inhibitors to modulate CBP-target interactions. The protocol described here uses a commercially available phage display library, in which random 12-mer peptides are displayed on filamentous M13 phages. The library was screened against the calcium-binding protein S100B.

PMID:
23296614
DOI:
10.1007/978-1-62703-230-8_14
[Indexed for MEDLINE]

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