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World J Stem Cells. 2012 Nov 26;4(11):110-6. doi: 10.4252/wjsc.v4.i11.110.

Umbilical cord blood mesenchymal stem cells protect amyloid-β42 neurotoxicity via paracrine.

Author information

  • 1Ju-Yeon Kim, Dong Hyun Kim, Ji Hyun Kim, Yoon Sun Yang, Wonil Oh, Jong Wook Chang, Biomedical Research Institute, R & D Center, MEDIPOST Co., Ltd. Seoul 137-874, South Korea.

Abstract

AIM:

To understand the neuroprotective mechanism of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) against amyloid-β42 (Aβ42) exposed rat primary neurons.

METHODS:

To evaluate the neuroprotective effect of hUCB-MSCs, the cells were co-cultured with Aβ42-exposed rat primary neuronal cells in a Transwell apparatus. To assess the involvement of soluble factors released from hUCB-MSCs in neuroprotection, an antibody-based array using co-cultured media was conducted. The neuroprotective roles of the identified hUCB-MSC proteins was assessed by treating recombinant proteins or specific small interfering RNAs (siRNAs) for each candidate protein in a co-culture system.

RESULTS:

The hUCB-MSCs secreted elevated levels of decorin and progranulin when co-cultured with rat primary neuronal cells exposed to Aβ42. Treatment with recombinant decorin and progranulin protected from Aβ42-neurotoxicity in vitro. In addition, siRNA-mediated knock-down of decorin and progranulin production in hUCB-MSCs reduced the anti-apoptotic effects of hUCB-MSC in the co-culture system.

CONCLUSION:

Decorin and progranulin may be involved in anti-apoptotic activity of hUCB-MSCs exposed to Aβ42.

KEYWORDS:

Anti-apoptosis; Aβ42; Decorin; Human umbilical cord blood-derived mesenchymal stem cells; Progranulin

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