Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr. 1990 Feb 23;525(2):349-57.

Determination of nornicotine in smokers' urine by gas chromatography following reductive alkylation to N'-propylnornicotine.

Author information

Department of Medicine, San Francisco General Hospital Medical Center, CA 94110.


A sensitive gas chromatographic assay has been developed to measure concentrations of nornicotine in human urine. A structural analogue, 5-methylnornicotine, is used as an internal standard. Both nornicotine and the internal standard were converted to the corresponding N'-propyl derivatives using a novel reductive alkylation procedure. The N'-propyl derivatives have good chromatographic properties, which allows quantitative measurement in the low nanograms per milliliter range. Concentrations of nornicotine in smokers' urine were quantitated and compared with the concentrations of nicotine and its metabolites, cotinine, nicotine-N-oxide and cotinine-N-oxide.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center