Format

Send to

Choose Destination
See comment in PubMed Commons below
J Cell Biol. 2013 Jan 7;200(1):21-30. doi: 10.1083/jcb.201210091. Epub 2012 Dec 31.

Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes.

Author information

  • 1Research Institute of Molecular Pathology, 1030 Vienna, Austria.

Abstract

Kinetochores are large protein complexes that link sister chromatids to the spindle and transduce microtubule dynamics into chromosome movement. In budding yeast, the kinetochore-microtubule interface is formed by the plus end-associated Dam1 complex and the kinetochore-resident Ndc80 complex, but how they work in combination and whether a physical association between them is critical for chromosome segregation is poorly understood. Here, we define structural elements required for the Ndc80-Dam1 interaction and probe their function in vivo. A novel ndc80 allele, selectively impaired in Dam1 binding, displayed growth and chromosome segregation defects. Its combination with an N-terminal truncation resulted in lethality, demonstrating essential but partially redundant roles for the Ndc80 N-tail and Ndc80-Dam1 interface. In contrast, mutations in the calponin homology domain of Ndc80 abrogated kinetochore function and were not compensated by the presence of Dam1. Our experiments shed light on how microtubule couplers cooperate and impose important constraints on structural models for outer kinetochore assembly.

PMID:
23277429
PMCID:
PMC3542791
DOI:
10.1083/jcb.201210091
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center